PE anti-human CD62L 304805 from BioLegend

Product Specs
ItemPE anti-human CD62L
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number304805
NCBI Gene AliasesCD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1
CloneDREG-56
IsotypeIgG1, Kappa
Quantity25 tests
ApplicationsFlow Cytometry (FC)
Conjugate/TagPE
ReactivityHuman
HostMouse
Antibody TypePrimary
NCBI Full Gene Nameselectin L
Formatµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal
TargetCD62L
ImmunogenConcentrated supernatant from PMA-activated human peripheral blood leukocytes

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Multimodal analysis of granulocytes, monocytes, and platelets in patients with cystic fibrosis before and after Elexacaftor–Tezacaftor–Ivacaftor treatmentFrontiers in ImmunologyJune 29, 2023Hanna Schmidt, Larissa Melina Höpfer, Lisa Wohlgemuth, Christiane Leonie Knapp, Adam Omar Khalaf Mohamed, Laura Stukan, Frederik Münnich, Dominik Hüsken, Alexander Sebastian Koller, Alexander Elias Paul Stratmann, et al.anti-mouse/human CD11b, dilution 1:3333, #101212, BioLegend), anti-CD62L (PE anti-human CD62L, dilution 1:400, #304806, BioLegend), anti-CD66b (APC-Cyanine7 anti-human CD66b, dilution 1:200, #305126, BioLegend), or corresponding

(2) Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitnessCell researchMay 1, 2023Chen J, Qiu S, Li W, Wang K, Zhang Y, Yang H, Liu B, Li G, Li L, Chen M, et al.eBioscience, 25-2239-42), anti-TIM-3-PerCP-Cy5.5 (1:800, Biolegend, 345016), anti-CD62L-PE (1:1000, Biolegend, 304806), anti-CD45RA-PE-Cy7 (1:1000, Biolegend, 304125), anti-Tox/Tox2-Alexa Fluor 647 (1:50, Cell Signaling Technology

(3) Combined Heterozygous Genetic Variations in Complement C2 and C8B: An Explanation for Multidimensional Immune Imbalance?Journal of Innate ImmunityMarch 1, 2023Marco Mannes, Rebecca Halbgebauer, Lisa Wohlgemuth, David Alexander Christian Christian Messerer, Susa Savukoski, Anke Schultze, Bettina Berger, Christiane Leonie Knapp, Christoph Q. Schmidt, Daniel Fürst, et al.FITC-conjugated, 315304, BioLegend, USA; isotype: 400108, BioLegend, USA), anti-CD62L (62.5 ng/mL, PE-conjugated, 304806, BioLegend, USA; isotype: 400112, BioLegend, USA), anti-CD16 (0.2 μg/mL, PerCP-conjugated, 302030, BioLegend,

(4) Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancerNature communicationsOctober 19, 2022Cao B, Liu M, Wang L, Zhu K, Cai M, Chen X, Feng Y, Yang S, Fu S, Zhi C, et al.206D), anti-human CD45RO-PerCP/Cy5.5 (Biolegend, #304222, clone UCHL1), anti-human CD62L-PE (Biolegend, #304806, clone DREG-56), anti-human CD68-APC (Biolegend, #333810, clone Y1/82 A), anti-human CD19-PE (Biolegend,

(5) RASA2 ablation in T cells boosts antigen sensitivity and long-term functionNatureSeptember 1, 2022Carnevale J, Shifrut E, Kale N, Nyberg WA, Blaeschke F, Chen YY, Li Z, Bapat SP, Diolaiti ME, O'Leary P, et al.CD366 (Tim-3) (Biolegend 345008), PE anti-human CD39 (Biolegend 328208), PE anti-human CD62L (Biolegend 304806), PE CD19 (Beckman Coulter IM1285U) and APC CD19 (Beckman Coulter IM2470U). For phospho-flow cytometry

(6) Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated ManufacturingFrontiers in Medical TechnologyMay 30, 2022Himavanth Gatla, Nicholas Uth, Yonatan Levinson, Ali Navaei, Alex Sargent, Senthil Ramaswamy, Inbar Friedrich Ben-Nunflow cytometry. Briefly, 300,000 cells were live-stained for the cell surface markers: CD62L (Biolegend Cat #304806), CD45RA (Biolegend Cat #304108), CD45RO (Biolegend Cat #304218), CD3 (BD Biosciences Cat #564713),

(7) RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunityNature communicationsApril 20, 2022Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, et al.317418), anti-CD8 (BioLegend, 344710), anti-CD45RA (BioLegend, 304112), and anti-CD62L (BioLegend, 304806), and analyzed by flow cytometry using BD FACSCelesta. In vitro expanded cells were stained by FITC-labeled

(8) Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1The Journal of clinical investigationJanuary 18, 2022Sung BY, Lin YH, Kong Q, Shah PD, Glick Bieler J, Palmer S, Weinhold KJ, Chang HR, Huang H, Avery RK, et al.

(9) The GSK3β-β-catenin-TCF1 pathway improves naive T cell activation in old adults by upregulating miR-181anpj Aging and Mechanisms of DiseaseDecember 1, 2021Zhongde Ye, Timothy M. Gould, Huimin Zhang, Jun Jin, Cornelia M. Weyand, Jörg J. Goronzyincluding CD4-APC (555349), CD69- PerCP-Cy5.5 (560738) and CD45RA-PE-Cy™7 and BioLegend including CD62L-PE (304806), CD25-APC (302610) and human IL-2 Alexa-Fluor-488 (500314). All antibodies for flow cytometry were

(10) The cell-surface 5′-nucleotidase CD73 defines a functional T memory cell subset that declines with ageCell ReportsNovember 9, 2021Fengqin Fang et al.AB_1595438 Anti-h CD8a (RPA-T8),Biolegend,Cat# 301016; RRID: AB_314134 Anti-h CD62L (DREG-56),Biolegend,Cat# 304806; RRID: AB_314466 Anti-h CD127 (A019D5),Biolegend,Cat# 351303; RRID: AB_10719960 Anti-h CD117 (104D2),Biolegend,Cat#

(11) Aged neutrophils form mitochondria-dependent vital NETs to promote breast cancer lung metastasisJournal for immunotherapy of cancerOctober 1, 2021Yang C, Wang Z, Li L, Zhang Z, Jin X, Wu P, Sun S, Pan J, Su K, Jia F, et al.HI30, #304014), CD11b (Clone ICRF44, #301322), CD66b (Clone G10F5, #305104), CD62L (Clone DREG-56, #304806), and CXCR4 (Clone 12G5; #306510, all from BioLegend). Samples were incubated in the dark for 20 min

(12) A novel inactivated whole-cell Pseudomonas aeruginosa vaccine that acts through the cGAS-STING pathwaySignal transduction and targeted therapyOctober 1, 2021Ma C, Ma X, Jiang B, Pan H, Liao X, Zhang L, Li W, Luo Y, Shen Z, Cheng X, et al.anti-CD8a-PE-Cy7 (Biolegend, 344750), anti-CD45RO-PerCP (Biolegend, 304251), and anti-CD62L-PE (Biolegend, 304805) antibodies after the treatment and analyzed using the Novocyto flow cytometer. Isotype controls were

(13) Rationally Designed Transgene-Encoded Cell-Surface Polypeptide Tag for Multiplexed Programming of CAR T-cell Synthetic OutputsCancer Immunology ResearchSeptember 1, 2021Adam J. Johnson, Jia Wei, James M. Rosser, Annette Künkele, Cindy A. Chang, Aquene N. Reid, Michael C. Jensencat. # 301040), CD45 (HI30; cat. #304028), CD45RO (UCHL1; cat. #983102), and CD62L (DREG-56; cat. #304806), and fluorochrome-conjugated streptavidin (cat. #405204) were obtained from BioLegend. Antibody isotype

(14) Activation of Neutrophil Granulocytes by Platelet-Activating Factor Is Impaired During Experimental SepsisFrontiers in ImmunologyMarch 16, 2021Stefan Hug et al.APC anti-mouse/human CD11b antibody (#101212, Biolegend, San Diego, USA) and 0.5 µg/ml PE anti-human CD62L antibody (#304806, Biolegend) for 5 min at room temperature. Cells were fixed using FACS Lysing Solution (BD, Heidelberg,

(15) Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammationNature communicationsFebruary 22, 2021Jones N, Blagih J, Zani F, Rees A, Hill DG, Jenkins BJ, Bull CJ, Moreira D, Bantan AIM, Cronin JG, et al.130-117-356) and anti-CCR2 (clone REA624; 130-109-595; all Miltenyi Biotec) and anti-CD62L (clone DREG-56; 304806; BioLegend). For mitochondria staining, cells were incubated with 100 nM MitoTracker Green (M7514; Thermo Fisher

(16) Arachidonic acid-regulated calcium signaling in T cells from patients with rheumatoid arthritis promotes synovial inflammationNature communicationsFebruary 10, 2021Ye Z, Shen Y, Jin K, Qiu J, Hu B, Jadhav RR, Sheth K, Weyand CM, Goronzy JJ.(560738), p-ERK (Thr202/Try204)-AF670 (561992), and p-SLP76 (Y128)-AF647 (558438)] or BioLegend [CD62L-PE (304806), IKAROS-AF647 (368404), and CD3-AF488 (30045)]. The LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit from

(17) In vitro OP9-DL1 co-culture and subsequent maturation in the presence of IL-21 generates tumor antigen-specific T cells with a favorable less-differentiated phenotype and enhanced functionalityOncoimmunologyJanuary 1, 2021Bonte S, de Munter S, Billiet L, Goetgeluk G, Ingels J, Jansen H, Pille M, de Cock L, Weening K, Taghon T, et al.(BD Biosciences, 551285); phycoerythrin (PE)-conjugated: CD7 (BioLegend, 343106), CD62L (BioLegend, 304806), CD95 (Miltenyi, 130–099-094), CD184 (BD Biosciences, 555974), CD279 (Miltenyi, 130–120-388), CD314

(18) Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM MicroscopySTAR protocolsJune 19, 2020Fan Z, Mikulski Z, McArdle S, Sundd P, Ley K.CD11b (FITC, clone ICRF44)",Biolegend,Cat# 301330 "Anti-human L-selectin (PE, DREG-56)",Biolegend,Cat# 304806 "Anti-human CD66b (FITC, clone G10F5)",Biolegend,Cat# 305104 "Anti-human Siglec-8 (PE, clone 7C9)",Biolegend,Cat#

(19) PSCA is a target of chimeric antigen receptor T cells in gastric cancerBiomarker researchJanuary 1, 2020Wu D, Lv J, Zhao R, Wu Z, Zheng D, Shi J, Lin S, Wang S, Wu Q, Long Y, et al.

(20) Animal-Free Human Whole Blood Sepsis Model to Study Changes in Innate ImmunityFrontiers in immunologyJanuary 1, 2020Messerer DAC, Vidoni L, Erber M, Stratmann AEP, Bauer JM, Braun CK, Hug S, Adler A, Nilsson Ekdahl K, Nilsson B, et al.California, USA), anti-CD14 (APC-Cy7, dilution 1:200 #301820, BioLegend), anti-CD62l (PE, dilution 1:33 #304806, BioLegend), anti-CD88 (APC, dilution 1:250 #344310, BioLegend), or corresponding isotype controls (all

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7/15/2019Anti-Human CD62L Antibody from BioLegend Works Well Gemma FouldsEasy-to-use antibody pack that is easily used in multi-colour assays.

PE anti-human CD62L from BioLegend

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PE anti-human CD62L from BioLegend

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